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人白介素6(IL6)酶聯(lián)免疫吸附檢測試劑盒

ELK1156

規格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻

Product name:	Human IL6(Interleukin 6) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
Assay Type:	Sandwich
Sensitivity:	3.2 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;
Uniprot ID:	P05231
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Clinica Chimica Acta

Predictive value of soluble fibrinogen-like protein 2 for survival in traumatic patients with sepsis

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researchsquare

GhLIP1, a lipoic acid synthase gene, negatively regulates leaf senescence in cotton

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Icariin protects neurons from endoplasmic reticulum stress-induced apoptosis after OGD/R injury via suppressing IRE1α-XBP1 signaling pathway

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Biomedicine & Pharmacotherapy

JNK downregulation improves olanzapine-induced insulin resistance by suppressing IRS1Ser307 phosphorylation and reducing inflammation

日期：2021-08-17 IF：6.529
Oxidative Medicine and Cellular Longevity

Vagus Nerve Stimulation Ameliorates Renal Ischemia-Reperfusion Injury through Inhibiting NF-κB Activation and iNOS Protein Expression

日期：2020-02-20 IF：6.543
Phytomedicine Plus

Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis

日期：2021-01-03 IF：-
Genes

Exosomes derived from miRNA-210 overexpressing bone marrow mesenchymal stem cells protect lipopolysaccharide induced chondrocytes injury via the NF-κB pathway

日期：2020-05-16 IF：3.688
Journal of Intensive Care

Low levels of CD72 and CD100 expression on circulating lymphocytes in immunosuppressive phase of sepsis is associated with mortality in septic patients

日期：2020-09-03 IF：3.953
Oxidative Medicine and Cellular Longevity

Serum Lipopolysaccharide Is Associated with the Recurrence of Atrial Fibrillation after Radiofrequency Ablation by Increasing Systemic Inflammation and Atrial Fibrosis

日期：2022-10-15 IF：7.31
Biochemical Pharmacology

Exosome modification to better alleviates endoplasmic reticulum stress induced chondrocyte apoptosis and osteoarthritis

日期：2022-11-23 IF：6.1
bioRxiv

Exosomes derived from BMSCs with miR-124-3p inhibitor protects against LPS-induced endometritis through regulation of DUSP6, p-p65 and p-ERK

日期：2023-02-02 IF：-
Journal of Clinical Medicine

Elevated Circulating Endocan Levels Are Associated with Increased Levels of Endothelial and Inflammation Factors in Postprandial Lipemia

日期：2023-02-06 IF：4.964
Cureus

An Evaluation of T-Regulatory Cells and Inflammatory Cytokines in Preeclampsia

日期：2023-08-12 IF：1.2
Radiation-induced non-neoplastic cells senescence promoting the proliferation and invasiveness of GBM through SASP/JAK2-STAT3 Pathway

bioRXiv

日期：2024-02-23 IF：3.8
bioRXiv

Radiation-induced non-neoplastic cells senescence promoting the proliferation and invasiveness of GBM through SASP/JAK2-STAT3 Pathway

日期：2024-02-23 IF：3.8

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
500.00	2.328	2.260
250.00	2.045	1.977
125.00	1.412	1.344
62.50	0.780	0.712
31.25	0.444	0.376
15.63	0.262	0.194
7.82	0.132	0.064
0.00	0.068	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL6 in samples.

Matrix	Recovery range	Average
serum(n=5)	97-105%	101%
EDTA plasma(n=5)	87-99%	92%
Heparin plasma(n=5)	88-107%	94%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.