免费久久无码精品一区二区,国产AV一区二区最新精品无删减,欧美久久男人的天堂,免费国产小视频国产

產(chǎn)品中心

人白介素1β(IL1b)酶聯(lián)免疫吸附檢測試劑盒

ELK1270

規格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00

下載說(shuō)明 ①

Overview 文獻

Product name:	Human IL1b(Interleukin 1 Beta) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-1B;IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
Assay Type:	Sandwich
Sensitivity:	5.8 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Uniprot ID:	P01584
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.

Frontiers in Oncology

Downregulation of hsa_circ_0001836 Induces Pyroptosis Cell Death in Glioma Cells via Epigenetically Upregulating NLRP1

日期：2021-10-20 IF：4.848
Life Sciences

Icariin protects neurons from endoplasmic reticulum stress-induced apoptosis after OGD/R injury via suppressing IRE1α-XBP1 signaling pathway

日期：2020-05-15 IF：5.037
Biomedicine & Pharmacotherapy

JNK downregulation improves olanzapine-induced insulin resistance by suppressing IRS1Ser307 phosphorylation and reducing inflammation

日期：2021-08-17 IF：6.529
Phytomedicine Plus

Inhibition of NOX4 by Cissus quadrangularis extract protects from Type 2 diabetes induced-steatohepatitis

日期：2021-01-03 IF：-
Journal of Intensive Care

Low levels of CD72 and CD100 expression on circulating lymphocytes in immunosuppressive phase of sepsis is associated with mortality in septic patients

日期：2020-09-03 IF：3.953
Bioengineered

Knockdown of lncRNA PCAT6 suppresses the growth of non-small cell lung cancer cells by inhibiting macrophages M2 polarization via miR-326/KLF1 axis

日期：2022-05-24 IF：3.269
Journal of Biomedical Science

Gut microbiota-mediated lysophosphatidylcholine generation promotes colitis in intestinal epithelium-specific Fut2 deficiency

日期：2021-06-15 IF：12.771
Drug Development Research

Knockdown of IGF2BP3 inhibits the tumorigenesis of gallbladder cancer and modifies tumor microenvironment

日期：2022-10-02 IF：5.004
Open Medicine

ANO6 (TMEM16F) inhibits gastrointestinal stromal tumor growth and induces ferroptosis

日期：2024-05-15 IF：2.1

標準曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.207	2.125
500.00	1.613	1.531
250.00	1.115	1.033
125.00	0.757	0.675
62.50	0.421	0.339
31.25	0.297	0.215
15.63	0.195	0.113
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL1b and the recovery rates were calculated by comparing the measured value to the expected amount of IL1b in samples.

Matrix	Recovery range	Average
serum(n=5)	92-105%	98%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	79-93%	86%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.